![]() ![]() Researches have been conducted to develop functional raw materials to improve physiological activity. These findings indicate that ERJ has high potential as an antioxidant agent for use in human and animal diets. Interestingly, these antioxidant activities of ERJ were stronger than those of non-treated RJ. Moreover, ERJ significantly increased the activity of the antioxidant enzyme superoxide dismutase (SOD) and the level of the antioxidant glutathione (GSH) in a dose-dependent manner. The antioxidant effect of ERJ was confirmed to be due to reduction of intracellular reactive oxidative species (ROS) and nitric oxide (NO) production in LPS-treated macrophages. The ERJ showed about 80% DPPH-radical scavenging activity at same concentration of ascorbic acid. ![]() The antioxidant activity of ERJ was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and chemical assays. Enzyme-treated royal jelly (ERJ), in which the RJ hydrolysates were converted into easy-to-absorb shorter chain monomers through the removal of two known allergen proteins, showed no difference in the content of ( E)-10-hydroxydec-2-enoicacid (10-HDA) or the freshness parameter and showed a significant increase in total free amino acid content. In this study, we investigated the antioxidant activity of royal jelly (RJ) hydrolysates obtained from three commercial proteases. Recently, research on the processing of raw functional materials with the aim of improving various physiological activities has been conducted. ![]()
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